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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C9orf171 Polyclonal antibody

C9orf171 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 24969-1-AP

Synonyms

Cilia- and flagella-associated protein 77, CFAP77

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C9orf171 Polyclonal antibody (24969-1-AP)

    WB analysis using 24969-1-AP

    Mouse testis tissue lysates were subjected to SDS PAGE followed by western blot with 24969-1-AP (C9orf171 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using C9orf171 Polyclonal antibody (24969-1-AP)

    IF Staining of mouse testis using 24969-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C9orf171 antibody (24969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using C9orf171 Polyclonal antibody (24969-1-AP)

    IF Staining of mouse testis using 24969-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C9orf171 antibody (24969-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using C9orf171 Polyclonal antibody (24969-1-AP)

    IF Staining of U-251 using 24969-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using C9orf171 antibody (24969-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24969-1-AP targets C9orf171 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf171 fusion protein Ag21797 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 171
Calculated molecular weight 320 aa, 36 kDa
Observed molecular weight33-36 kDa
GenBank accession numberBC118593
Gene symbol C9orf171
Gene ID (NCBI) 389799
RRIDAB_3085763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C9orf171 antibody 24969-1-APDownload protocol
IF protocol for C9orf171 antibody 24969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols