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Species Reactivity

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  • Featured Product
  • KD/KO Validated

C9orf41/CARNMT1 Polyclonal antibody

C9orf41/CARNMT1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24068-1-AP

Synonyms

C9orf41, EC:2.1.1.22, Carnosine N-methyltransferase, CARNMT1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using C9orf41/CARNMT1 Polyclonal antibody (24068-1-AP)

    WB analysis of HepG2 using 24068-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using C9orf41/CARNMT1 Polyclonal antibody (24068-1-AP)

    WB analysis of HeLa using 24068-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using C9orf41/CARNMT1 Polyclonal antibody (24068-1-AP)

    WB analysis of MCF-7 using 24068-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using C9orf41/CARNMT1 Polyclonal antibody (24068-1-AP)

    IF Staining of HepG2 using 24068-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf41/CARNMT1 antibody (24068-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24068-1-AP targets C9orf41/CARNMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf41/CARNMT1 fusion protein Ag21268 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 41
Calculated molecular weight 409 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC034033
Gene symbol C9orf41
Gene ID (NCBI) 138199
RRIDAB_2879425
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The enzyme CARNMT1 (carnosine N-methyltransferase 1, also known as C9orf41), conserved from yeast to human, was identified as an N1 position-specific methyltransferase that catalyzes the conversion of carnosine to anserine (β-alanyl-N-π-methyl-L-histidine).

Protocols

Product Specific Protocols
WB protocol for C9orf41/CARNMT1 antibody 24068-1-APDownload protocol
IF protocol for C9orf41/CARNMT1 antibody 24068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Genes Dev

Histidine N1-position-specific methyltransferase CARNMT1 targets C3H zinc finger proteins and modulates RNA metabolism

Authors - Tadahiro Shimazu
  • KO Validated
View Article