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  • Featured Product
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C9orf64 Polyclonal antibody

C9orf64 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27797-1-AP

Synonyms

Queuosine-nucleotide N-glycosylase/hydrolase, Queuosine 5'-phosphate N-glycosylase/hydrolase, Queuine salvage protein QNG1, Q-nucleotide N-glycosylase 1, QNG1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using C9orf64 Polyclonal antibody (27797-1-AP)

    WB analysis of HeLa using 27797-1-AP

    WB result of C9orf64 antibody (27797-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C9orf64 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using C9orf64 Polyclonal antibody (27797-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27797-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27797-1-AP (C9orf64 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27797-1-AP targets C9orf64 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf64 fusion protein Ag23069 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 64
Calculated molecular weight 341 aa, 39 kDa
Observed molecular weight34-39 kDa
GenBank accession numberBC004407
Gene symbol C9orf64
Gene ID (NCBI) 84267
RRIDAB_2918132
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for C9orf64 antibody 27797-1-APDownload protocol
IHC protocol for C9orf64 antibody 27797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols