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KD/KO Validated

C9orf72 Polyclonal antibody

Name: C9orf72 Polyclonal antibody
Brand: Proteintech
SKU: 22637-1-AP

C9orf72 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22637-1-AP

Synonyms

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of rat brain using 22637-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 22637-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22637-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 22637-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Sp2/0 using 22637-1-AP
Sp2/0 cells were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 22637-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of transfected cells using 22637-1-AP
WB result of anti-C9ORF72 (22637-1-AP) with Variant1 and 2 transfected. (by Dr. Sarah Mueller).
IP experiment of SH-SY5Y using 22637-1-AP
IP result of anti-C9orf72 (IP:22637-1-AP, 3ug; Detection:22637-1-AP 1:300) with SH-SY5Y cells lysate 2000ug.
IHC staining of human gliomas using 22637-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22637-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 22637-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22637-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 22637-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22637-1-AP (C9orf72 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 22637-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22637-1-AP (C9orf72 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human gliomas using 22637-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22637-1-AP (C9orf72 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human gliomas using 22637-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22637-1-AP (C9orf72 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of SH-SY5Y using 22637-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9orf72 antibody (22637-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Cat No. 22637-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	C2C12 cells, transfected cells, HeLa cells, Sp2/0 cells, SH-SY5Y cells, rat brain tissue, mouse testis tissue, HEK-293 cells, NIH/3T3 cells
Positive IP detected in	SH-SY5Y cells
Positive IHC detected in	human gliomas tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 17 publications below
WB	See 31 publications below
IHC	See 2 publications below
IF	See 9 publications below
CoIP	See 2 publications below

Product Information

22637-1-AP targets C9orf72 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, caenorhabditis elegans
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	C9orf72 fusion protein Ag18326 相同性解析による交差性が予測される生物種
Full Name	chromosome 9 open reading frame 72
Calculated molecular weight	481 aa, 54 kDa
Observed molecular weight	50-54 kDa
GenBank accession number	BC020851
Gene symbol	C9orf72
Gene ID (NCBI)	203228
RRID	AB_10953528
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. It has been reported that C9orf72 forms a complex with Cofilin and other actin binding proteins and 22637-1-AP antibody detects the complex bands around 43 and 68-72 kDa in SDS-PAGE.(PMID: 27723745)

Protocols

Product Specific Protocols
WB protocol for C9orf72 antibody 22637-1-AP	Download protocol
IHC protocol for C9orf72 antibody 22637-1-AP	Download protocol
IF protocol for C9orf72 antibody 22637-1-AP	Download protocol
IP protocol for C9orf72 antibody 22637-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Science C9orf72 is required for proper macrophage and microglial function in mice. Authors - J G O'Rourke KO Validated View Article
human	wb	Nat Med Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons. Authors - Yingxiao Shi View Article
human,mouse	WB,CoIP	Cell Metab C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly. Authors - Tao Wang KO Validated View Article
human,mouse	WB	Nat Neurosci Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72. Authors - Qiang Zhu KO Validated View Article
human	WB	Nat Commun C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy Authors - Shen Zhang KO Validated View Article
mouse	WB	Sci Transl Med Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease. Authors - Aaron Burberry KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C9orf72 Polyclonal antibody

C9orf72 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of rat brain using 22637-1-AP

WB analysis using 22637-1-AP

WB analysis of HeLa using 22637-1-AP

WB analysis of SH-SY5Y using 22637-1-AP

WB analysis of Sp2/0 using 22637-1-AP

WB analysis of mouse testis using 22637-1-AP

WB analysis of transfected cells using 22637-1-AP

IP experiment of SH-SY5Y using 22637-1-AP

IHC staining of human gliomas using 22637-1-AP

IHC staining of human gliomas using 22637-1-AP

IHC staining of human testis using 22637-1-AP

IHC staining of human testis using 22637-1-AP

IHC staining of human gliomas using 22637-1-AP

IHC staining of human gliomas using 22637-1-AP

IF Staining of SH-SY5Y using 22637-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

C9orf72 is required for proper macrophage and microglial function in mice.

Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.

Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72.

C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy

Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.

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