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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C9orf72 Monoclonal antibody, PBS Only

C9orf72 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D2H6

Cat no : 66140-1-PBS

Synonyms

C9orf72, C9RANT

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    WB analysis of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using C9orf72 Monoclonal antibody (66140-1-Ig)

    WB analysis of C6 using 66140-1-Ig (same clone as 66140-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using C9orf72 Monoclonal antibody (66140-1-Ig)

    WB analysis of Neuro-2a using 66140-1-Ig (same clone as 66140-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of C6 cells using C9orf72 Monoclonal antibody (66140-1-Ig)

    IP experiment of C6 using 66140-1-Ig (same clone as 66140-1-PBS)

    IP result of anti-C9orf72 (IP:66140-1-Ig, 4ug; Detection:66140-1-Ig 1:1000) with C6 cells lysate 1320ug. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using C9orf72 Monoclonal antibody (66140-1-Ig)

    IF Staining of SH-SY5Y using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunofluorescent analysis of SH-SY5Y cells using 66140-1-Ig (C9orf72 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66140-1-PBS targets C9orf72 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen C9orf72 fusion protein Ag21080 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 72
Calculated molecular weight 481 aa, 54 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC020851
Gene symbol C9orf72
Gene ID (NCBI) 203228
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.