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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C9orf72 Monoclonal antibody, PBS Only

C9orf72 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D2H6

Cat no : 66140-1-PBS

Synonyms

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 3D2H6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    WB analysis of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using C9orf72 Monoclonal antibody (66140-1-Ig)

    WB analysis of C6 using 66140-1-Ig (same clone as 66140-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Neuro-2a cells using C9orf72 Monoclonal antibody (66140-1-Ig)

    WB analysis of Neuro-2a using 66140-1-Ig (same clone as 66140-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of C6 cells using C9orf72 Monoclonal antibody (66140-1-Ig)

    IP experiment of C6 using 66140-1-Ig (same clone as 66140-1-PBS)

    IP result of anti-C9orf72 (IP:66140-1-Ig, 4ug; Detection:66140-1-Ig 1:1000) with C6 cells lysate 1320ug. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using C9orf72 Monoclonal antibody (66140-1-Ig)

    IHC staining of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using C9orf72 Monoclonal antibody (66140-1-Ig)

    IF Staining of SH-SY5Y using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9orf72 antibody (66140-1-Ig, Clone: 3D2H6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66140-1-PBS targets C9orf72 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen C9orf72 fusion protein Ag21080 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 72
Calculated molecular weight 481 aa, 54 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC020851
Gene symbol C9orf72
Gene ID (NCBI) 203228
RRIDAB_2784547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.