C9orf72 Monoclonal antibody
C9orf72 Monoclonal Antibody for WB, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
2F9A2
Cat no : 67824-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig brain tissue, HEK-293 cells, U-87 MG cells, Neuro-2a cells, PC-12 cells, C6 cells, rat brain tissue, mouse brain tissue |
Positive FC (Intra) detected in | HEK-293T cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67824-1-Ig targets C9orf72 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | C9orf72 fusion protein Ag21080 相同性解析による交差性が予測される生物種 |
Full Name | chromosome 9 open reading frame 72 |
Calculated molecular weight | 481 aa, 54 kDa |
Observed molecular weight | 50 kDa |
GenBank accession number | BC020851 |
Gene symbol | C9orf72 |
Gene ID (NCBI) | 203228 |
RRID | AB_2918586 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for C9orf72 antibody 67824-1-Ig | Download protocol |
FC protocol for C9orf72 antibody 67824-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |