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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

C9orf72 Monoclonal antibody

C9orf72 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F9A2

Cat no : 67824-1-Ig

Synonyms

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 2F9A2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C9orf72 Monoclonal antibody (67824-1-Ig)

    WB analysis using 67824-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of Neuro-2a cells using C9orf72 Monoclonal antibody (67824-1-Ig)

    WB analysis of Neuro-2a using 67824-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using C9orf72 Monoclonal antibody (67824-1-Ig)

    WB analysis of PC-12 using 67824-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using C9orf72 Monoclonal antibody (67824-1-Ig)

    WB analysis of C6 using 67824-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using C9orf72 Monoclonal antibody (67824-1-Ig)

    WB analysis of pig brain using 67824-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using C9orf72 Monoclonal antibody (67824-1-Ig)

    WB analysis of rat brain using 67824-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using C9orf72 Monoclonal antibody (67824-1-Ig)

    WB analysis of mouse brain using 67824-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using C9orf72 Monoclonal antibody (67824-1-Ig)

    WB analysis of HEK-293 using 67824-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-87 MG cells using C9orf72 Monoclonal antibody (67824-1-Ig)

    WB analysis of U-87 MG using 67824-1-Ig

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using C9orf72 Monoclonal antibody (67824-1-Ig)

    IHC staining of mouse lung using 67824-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse intestine using C9orf72 Monoclonal antibody (67824-1-Ig)

    IHC staining of mouse intestine using 67824-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using C9orf72 Monoclonal antibody (67824-1-Ig)

    IHC staining of rat kidney using 67824-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of HEK-293T cells using C9orf72 Monoclonal antibody (67824-1-Ig)

    FC experiment of HEK-293T using 67824-1-Ig

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human C9orf72 (67824-1-Ig, Clone:2F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inpig brain tissue, HEK-293 cells, U-87 MG cells, Neuro-2a cells, PC-12 cells, C6 cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse lung tissue, mouse intestine, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67824-1-Ig targets C9orf72 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen C9orf72 fusion protein Ag21080 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 72
Calculated molecular weight 481 aa, 54 kDa
Observed molecular weight50 kDa
GenBank accession numberBC020851
Gene symbol C9orf72
Gene ID (NCBI) 203228
RRIDAB_2918586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.

Protocols

Product Specific Protocols
WB protocol for C9orf72 antibody 67824-1-IgDownload protocol
IHC protocol for C9orf72 antibody 67824-1-IgDownload protocol
FC protocol for C9orf72 antibody 67824-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols