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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C9orf89 Recombinant antibody (85357-1-RR)

    WB analysis using 85357-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85357-1-RR (C9orf89 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C9orf89 Recombinant antibody (85357-1-RR)

    IF Staining of A431 using 85357-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C9orf89 antibody (85357-1-RR, Clone: 242638F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85357-1-RR

    Affinity and Kinetic Characterization of 85357-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85357-1-RR against Human C9orf89 were performed. The affinity constant is 0.756 nM.

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C9orf89 Recombinant antibody

C9orf89 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85357-1-RR
Clone No.242638F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Caspase recruitment domain-containing protein 19, CARD19, BinCARD, Bcl10-interacting CARD protein, 242638F7

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, C2C12 cells, H9C2 cells, rat kidney tissue, mouse kidney tissue, human placenta tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85357-1-RR targets C9orf89 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C9orf89 fusion protein Ag14084 相同性解析による交差性が予測される生物種
Full Name chromosome 9 open reading frame 89
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight15-21 kDa
GenBank accession numberBC004500
Gene Symbol C9orf89
Gene ID (NCBI) 84270
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96LW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C9orf89 (also known as CARD19 or BinCARD) is a mitochondria-localized protein that inhibits BCL10-induced NF-κB activation mainly through its caspase recruitment structural domain (CARD). It plays a role in T cell receptor (TCR) signaling, but deletion of endogenous CARD19 has a lesser effect on Bcl10-dependent NF-κB activation. In addition, C9orf89 is expressed in multiple cell types and is involved in the regulation of inflammatory and immune responses.

Protocols

Product Specific Protocols
WB protocol for C9orf89 antibody 85357-1-RRDownload protocol
IF protocol for C9orf89 antibody 85357-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols