Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, mouse liver tissue, rat testis tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
26034-1-AP targets SPACA9 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SPACA9 fusion protein Ag23320 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 9 open reading frame 9 |
| Observed molecular weight | 18 kDa, 30 kDa |
| GenBank accession number | BC012940 |
| Gene Symbol | SPACA9 |
| Gene ID (NCBI) | 11092 |
| RRID | AB_2880345 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96E40 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The function of C9orf9 remains largely unknown. Catalog#26034-1-AP is a rabbit polyclonal antibody raised against the full-length of human C9orf9. Two isoforms were produced by alternative splicing with predicted MW of 19 and 26 kDa (UniProt). Two bands at 16 kDa and 30 kDa could be detected using the present rabbit polyclonal antibody 26034-1-AP.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPACA9 antibody 26034-1-AP | Download protocol |
| IF protocol for SPACA9 antibody 26034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Biol CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement | ||
Cell Mol Life Sci CCDC146 is required for sperm flagellum biogenesis and male fertility in mice |