Validation Data Gallery

Filter: AllWBIHC

WB analysis of human kidney using 15435-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 15435-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 15435-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

CA11 Polyclonal antibody

CA11 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15435-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CA RP II, CA RP XI, CA XI, CA11, carbonic anhydrase XI, CARP 2, CARP XI, CARP2, CARPX1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human kidney tissue
Positive IHC detected in	human brain tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15435-1-AP targets CA11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CA11 fusion protein Ag7712 相同性解析による交差性が予測される生物種
Full Name	carbonic anhydrase XI
Calculated molecular weight	36 kDa
Observed molecular weight	36 kda, 45 kDa
GenBank accession number	BC002662
Gene Symbol	CA11
Gene ID (NCBI)	770
RRID	AB_2065700
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75493
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CA11(Carbonic anhydrase-related protein 11) is also named as CARP2, CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).

Protocols

Product Specific Protocols
WB protocol for CA11 antibody 15435-1-AP	Download protocol
IHC protocol for CA11 antibody 15435-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Target

Host

Species Reactivity

Species Reactivity

Conjugate