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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using CA13 Polyclonal antibody (16696-1-AP)

    WB analysis of NIH/3T3 using 16696-1-AP (same clone as 16696-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16696-1-AP (CA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using CA13 Polyclonal antibody (16696-1-AP)

    WB analysis of mouse skeletal muscle using 16696-1-AP (same clone as 16696-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16696-1-AP (CA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human placenta using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human placenta using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human testis using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human testis using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human spleen using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human spleen using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human heart using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human heart using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human kidney using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human kidney using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human spleen using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human spleen using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human spleen using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human spleen using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16696-1-AP (CA13 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human kidney using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CA13 Polyclonal antibody (16696-1-AP)

    IHC staining of human kidney using 16696-1-AP (same clone as 16696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CA13 Polyclonal antibody (16696-1-AP)

    IF Staining of NIH/3T3 using 16696-1-AP (same clone as 16696-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CA13 antibody (16696-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CA13 Polyclonal antibody (16696-1-AP)

    IF Staining of NIH/3T3 using 16696-1-AP (same clone as 16696-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 16696-1-AP (CA13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16696-1-PBS in a different storage buffer formulation.

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CA13 Polyclonal antibody, PBS Only

CA13 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16696-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16696-1-PBS targets CA13 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA13 fusion protein Ag10086 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase XIII
Calculated molecular weight 262 aa, 29 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC052602
Gene Symbol CA13
Gene ID (NCBI) 377677
RRIDAB_1850972
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1Q1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze the reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic protein that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).