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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CA3 Monoclonal antibody (66608-1-Ig)

    WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CA3 Monoclonal antibody (66608-1-Ig)

    WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CA3 Monoclonal antibody (66608-1-Ig)

    IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CA3 Monoclonal antibody (66608-1-Ig)

    IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using CA3 Monoclonal antibody (66608-1-Ig)

    IF Staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CA3 antibody (66608-1-Ig, Clone: 3C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

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CA3 Monoclonal antibody, PBS Only

CA3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66608-1-PBS
Clone No.3C10A2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Carbonic anhydrase III, Car3, CA-III, CAIII, CA III

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66608-1-PBS targets CA3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CA3 fusion protein Ag7513 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase III, muscle specific
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC004897
Gene Symbol CA3
Gene ID (NCBI) 761
RRIDAB_2881968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07451
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).