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Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using CA6 Monoclonal antibody (66909-1-Ig)

    WB analysis of human saliva using 66909-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 66909-1-Ig (CA6 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Carbonic Anhydrase 6/CA6 Monoclonal antibody

Carbonic Anhydrase 6/CA6 Monoclonal Antibody for WB, ELISA
Cat No. 66909-1-Ig
Clone No.1C8C7

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, ELISA

Synonyms

CA6, CA-VI, Carbonic anhydrase 6, Carbonate dehydratase VI, CA VI

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman saliva tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66909-1-Ig targets Carbonic Anhydrase 6/CA6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Carbonic Anhydrase 6/CA6 fusion protein Ag24274 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase VI
Calculated molecular weight 35 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC034350
Gene Symbol CA6
Gene ID (NCBI) 765
RRIDAB_2882236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23280
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA6 (Carbonic anhydrase VI ) is a member of CA family proteins that play a central role in pH regulation and electrolyte balance. CA6 is also known as gustin, is a zinc-containing secreted protein which catalyzes the hydration of carbon hydroxide in saliva. CA6 is specifically expressed in the salivary gland of a number of mammalian species (PMID:28423504). The amino acid sequences are highly conserved across the species. And it was reported that decreasing of CA6 protein was associated with loss of taste and pathological morphology of taste buds (PMID: 9784398).

Protocols

Product Specific Protocols
WB protocol for Carbonic Anhydrase 6/CA6 antibody 66909-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols