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Carbonic Anhydrase IX/CA9 Polyclonal antibody

Carbonic Anhydrase IX/CA9 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 11071-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

CAIX, CA9, carbonic anhydrase IX, Carbonate dehydratase IX, CA-IX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, mouse brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman stomach tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11071-1-AP targets Carbonic Anhydrase IX/CA9 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carbonic Anhydrase IX/CA9 fusion protein Ag1540 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase IX
Calculated molecular weight 459 aa, 50 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC014950
Gene Symbol CA9
Gene ID (NCBI) 768
RRIDAB_2066528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular. Western blotting detected CA9 protein at a molecular weight of 70 kDa (PMID: 31819036).

Protocols

Product Specific Protocols
WB protocol for Carbonic Anhydrase IX/CA9 antibody 11071-1-APDownload protocol
IHC protocol for Carbonic Anhydrase IX/CA9 antibody 11071-1-APDownload protocol
IP protocol for Carbonic Anhydrase IX/CA9 antibody 11071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Proc Natl Acad Sci U S A

CRLX101 nanoparticles localize in human tumors and not in adjacent, nonneoplastic tissue after intravenous dosing.

Authors - Andrew J Clark
humanWB

Cell Commun Signal

KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma

Authors - Mao Shen
humanWB

Biomed Pharmacother

Para-toluenesulfonamide, a novel potent carbonic anhydrase inhibitor, improves hypoxia-induced metastatic breast cancer cell viability and prevents resistance to αPD-1 therapy in triple-negative breast cancer

Authors - Hsin-Yuan Chen
human,mouseWB,IHC,IF

Oxid Med Cell Longev

Carbonic Anhydrase IX Controls Vulnerability to Ferroptosis in Gefitinib-Resistant Lung Cancer

Authors - Chen Zhang
mouseIHC

Oxid Med Cell Longev

Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer.

Authors - Wei Zou
mouseIHC,IF

Eur J Nucl Med Mol Imaging

In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT.

Authors - Wenhui Huang
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