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CA9 Polyclonal antibody
CA9 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11443-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11443-1-AP targets CA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CA9 fusion protein Ag1963 相同性解析による交差性が予測される生物種 |
| Full Name | carbonic anhydrase IX |
| Calculated molecular weight | 459 aa, 50 kDa |
| Observed molecular weight | 50-60 kDa |
| GenBank accession number | BC014950 |
| Gene symbol | CA9 |
| Gene ID (NCBI) | 768 |
| RRID | AB_2243631 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CA9 antibody 11443-1-AP | Download protocol |
| IHC protocol for CA9 antibody 11443-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Emma (Verified Customer) (10-28-2022) | Western blot, 30 µg lysate per well. Glioblastoma stem-like cells were cultured as small speroids and treated with CoCl2 to stabilise HIF-1a, or as medium or XL speroids (no CoCl2 treatment). There were are a few bands visible on the blot, but CoCl2 induced a band at around 60 kDa, and XL speroids which have a hypoxic core also resulted in a heavy band at the same height. See also: https://www.nature.com/articles/s41389-022-00433-3#Sec18 Figure S5 and supplementary methods for WB details.
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