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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CA9 Polyclonal antibody (11443-1-AP)

    WB analysis of mouse brain using 11443-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11443-1-AP (CA IX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CA9 Polyclonal antibody (11443-1-AP)

    WB analysis of A549 using 11443-1-AP

    WB result of CA9 antibody (11443-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA9 transfected A549 cells.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CA9 Polyclonal antibody (11443-1-AP)

    IHC staining of human stomach cancer using 11443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11443-1-AP (CA9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CA9 Polyclonal antibody (11443-1-AP)

    IHC staining of human stomach cancer using 11443-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11443-1-AP (CA9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO Validated

CA9 Polyclonal antibody

CA9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11443-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, A549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11443-1-AP targets CA9 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA9 fusion protein Ag1963 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase IX
Calculated molecular weight 459 aa, 50 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC014950
Gene Symbol CA9
Gene ID (NCBI) 768
RRIDAB_2243631
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.

Protocols

Product Specific Protocols
WB protocol for CA9 antibody 11443-1-APDownload protocol
IHC protocol for CA9 antibody 11443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols