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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CA9 Recombinant antibody

CA9 Recombinant Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241573A4

Cat no : 84233-1-RR

Synonyms

CAIX, carbonic anhydrase IX, Carbonate dehydratase IX, CA-IX, CA IX

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse stomach tissue using CA9 Recombinant antibody (84233-1-RR)

    IHC staining of mouse stomach using 84233-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84233-1-RR (CA9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using CA9 Recombinant antibody (84233-1-RR)

    IHC staining of mouse stomach using 84233-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84233-1-RR (CA9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84233-1-RR is CA9 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CA9 fusion protein Ag36941 相同性解析による交差性が予測される生物種
Full Name carbonic anhydrase IX
Calculated molecular weight 459 aa, 50 kDa
GenBank accession numberBC014950
Gene symbol CA9
Gene ID (NCBI) 768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular. (PMID: 31819036).

Protocols

Product Specific Protocols
IHC protocol for CA9 antibody 84233-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols