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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CABLES1 Polyclonal antibody

CABLES1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13464-1-AP

Synonyms

Ik3-1, HsT2563, CABLES

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CABLES1 Polyclonal antibody (13464-1-AP)

    WB analysis using 13464-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13464-1-AP (CABLES1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using CABLES1 Polyclonal antibody (13464-1-AP)

    IHC staining of human colon using 13464-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13464-1-AP (CABLES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CABLES1 Polyclonal antibody (13464-1-AP)

    IHC staining of mouse pancreas using 13464-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13464-1-AP (CABLES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CABLES1 Polyclonal antibody (13464-1-AP)

    IF Staining of MCF-7 using 13464-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CABLES1 antibody (13464-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CABLES1 Polyclonal antibody (13464-1-AP)

    IF Staining of HeLa using 13464-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CABLES1 antibody (13464-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inCOLO 205 cells, HT-29 cells, SH-SY5Y cells
Positive IHC detected inhuman colon tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13464-1-AP targets CABLES1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CABLES1 fusion protein Ag4251 相同性解析による交差性が予測される生物種
Full Name Cdk5 and Abl enzyme substrate 1
Calculated molecular weight 568 aa, 63 kDa
Observed molecular weight68 kDa, 42 kDa
GenBank accession numberBC037218
Gene symbol CABLES1
Gene ID (NCBI) 91768
RRIDAB_3669163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK5 and ABL1 enzyme substrate 1 (CABLES1) is a novel Cdk2, Cdk3, and Cdk5 binding protein, which acts as a link between the Cdks and nonreceptor tyrosine kinases and regulates the activity of Cdks by enhancing their Y15 phosphorylation. CABLES1 is a candidate tumor suppressor that negatively regulates cell growth by inhibiting cyclin-dependent kinases.

Protocols

Product Specific Protocols
WB protocol for CABLES1 antibody 13464-1-APDownload protocol
IHC protocol for CABLES1 antibody 13464-1-APDownload protocol
IF protocol for CABLES1 antibody 13464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols