Validation Data Gallery
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Tested Applications
| Positive WB detected in | human testis tissue, mouse testis tissue |
| Positive IHC detected in | human testis tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 3 publications below |
Product Information
12351-1-AP targets CABYR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CABYR fusion protein Ag3012 相同性解析による交差性が予測される生物種 |
| Full Name | calcium binding tyrosine-(Y)-phosphorylation regulated |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 53 kDa, 86-90 kDa |
| GenBank accession number | BC011996 |
| Gene Symbol | CABYR |
| Gene ID (NCBI) | 26256 |
| RRID | AB_2069257 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75952 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CABYR antibody 12351-1-AP | Download protocol |
| IHC protocol for CABYR antibody 12351-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Genet Genomics Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice | ||
J Cell Sci CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.
| ||
J Cell Sci Radial spoke head 6 homolog A is required for sperm flagellum formation and male fertility in mice. | ||
Nat Commun Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella. | ||