Featured Product
KD/KO Validated

CABYR Polyclonal antibody

Name: CABYR Polyclonal antibody
Brand: Proteintech
SKU: 12351-1-AP

CABYR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12351-1-AP

Synonyms

CABYR, Calcium binding protein 86, Cancer/testis antigen 88, CBP86, CT88, Fibrousheathin 2, Fibrousheathin II, FSP 2, FSP2

Validation Data Gallery

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AllWBIHC

WB analysis of human testis using 12351-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 12351-1-AP (CABYR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12351-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12351-1-AP (CABYR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 12351-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12351-1-AP (CABYR Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 12351-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12351-1-AP (CABYR Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human pancreas cancer using 12351-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12351-1-AP (CABYR antibody) at dilution of 1:50 (under 25x lens).
IHC staining of human pancreas cancer using 12351-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12351-1-AP (CABYR antibody) at dilution of 1:50 (under 25x lens).

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Cat No. 12351-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	human testis tissue, mouse testis tissue
Positive IHC detected in	human testis tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 3 publications below

Product Information

12351-1-AP targets CABYR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CABYR fusion protein Ag3012 相同性解析による交差性が予測される生物種
Full Name	calcium binding tyrosine-(Y)-phosphorylation regulated
Calculated molecular weight	53 kDa
Observed molecular weight	53 kDa, 86-90 kDa
GenBank accession number	BC011996
Gene symbol	CABYR
Gene ID (NCBI)	26256
RRID	AB_2069257
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CABYR antibody 12351-1-AP	Download protocol
IHC protocol for CABYR antibody 12351-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	J Genet Genomics Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice Authors - Yaling Wang View Article
MOUSE	WB	J Cell Sci CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. Authors - Samantha A M Young KO Validated View Article
mouse	IF	J Cell Sci Radial spoke head 6 homolog A is required for sperm flagellum formation and male fertility in mice. Authors - Ferheen Abbasi View Article
human,mouse	IF	Clin Genet Biallelic mutations in PMFBP1 cause acephalic spermatozoa. Authors - Yan-Wei Sha View Article
human	WB	Nat Commun Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella. Authors - Ying Shen View Article
	WB	Animals (Basel) Comparative Study on the Sperm Proteomes of Horses and Donkeys Authors - Hong Ren View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CABYR Polyclonal antibody

CABYR Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of human testis using 12351-1-AP

WB analysis of mouse testis using 12351-1-AP

IHC staining of human testis using 12351-1-AP

IHC staining of human testis using 12351-1-AP

IHC staining of human pancreas cancer using 12351-1-AP

IHC staining of human pancreas cancer using 12351-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa.

Radial spoke head 6 homolog A is required for sperm flagellum formation and male fertility in mice.

Biallelic mutations in PMFBP1 cause acephalic spermatozoa.

Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella.

Comparative Study on the Sperm Proteomes of Horses and Donkeys