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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CACNA1B Polyclonal antibody (30625-1-AP)

    WB analysis using 30625-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30625-1-AP (CACNA1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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CACNA1B Polyclonal antibody

CACNA1B Polyclonal Antibody for WB, ELISA
Cat No. 30625-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Cav2.2, Calcium channel, L type, alpha-1 polypeptide isoform 5, CACNN, CACNL1A5, CACH5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30625-1-AP targets CACNA1B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACNA1B fusion protein Ag33516 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, N type, alpha 1B subunit
Calculated molecular weight262 kDa
Observed molecular weight200 kDa
GenBank accession numberNM_000718
Gene Symbol CACNA1B
Gene ID (NCBI) 774
RRIDAB_3669741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00975
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CACNA1B, also named CACH5, CACNL1A5, and BIII, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division, and cell death. CACNA1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). CACNA1B may play a role in the directed migration of immature neurons.

Protocols

Product Specific Protocols
WB protocol for CACNA1B antibody 30625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols