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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CACNA1S Polyclonal antibody

CACNA1S Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22279-1-AP

Synonyms

CACH1, CACN1, CACNA1S, CACNL1A3, Cav1.1, CCHL1A3, HOKPP, hypoPP, MHS5

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using CACNA1S Polyclonal antibody (22279-1-AP)

    WB analysis of human brain using 22279-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CACNA1S Polyclonal antibody (22279-1-AP)

    WB analysis of human brain using 22279-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22279-1-AP (CACNA1S antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CACNA1S Polyclonal antibody (22279-1-AP)

    IHC staining of human skeletal muscle using 22279-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CACNA1S Polyclonal antibody (22279-1-AP)

    IHC staining of human skeletal muscle using 22279-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22279-1-AP (CACNA1S Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22279-1-AP targets CACNA1S in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, L type, alpha 1S subunit
Calculated molecular weight 212 kDa
Observed molecular weight 200-220 kDa
GenBank accession numberNM_000069
Gene symbol CACNA1S
Gene ID (NCBI) 779
RRIDAB_2879058
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CACNA1S, also named as CACH1, CACN1 and CACNL1A3, belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family and CACNA1S subfamily. Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. CACNA1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. The antibody is specific to CACNA1S.

Protocols

Product Specific Protocols
WB protocol for CACNA1S antibody 22279-1-APDownload protocol
IHC protocol for CACNA1S antibody 22279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Reproduction

Deep RNA sequencing analysis of syncytialization-related genes during BeWo cell fusion.

Authors - Ru Zheng
View Article
humanWB

bioRxiv

Modulation of blood-tumor barrier transcriptional programs improves intra-tumoral drug delivery and potentiates chemotherapy in GBM

Authors - Jorge L Jimenez-Macias
View Article