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CACNA2D2 Polyclonal antibody

CACNA2D2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22145-1-AP

Synonyms

CACNA2D, CACNA2D2, KIAA0558, LUAC11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using CACNA2D2 Polyclonal antibody (22145-1-AP)

    WB analysis of SH-SY5Y using 22145-1-AP

    WB result of CACNA2D2 antibody (22145-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CACNA2D2 transfected SH-SY5Y cells.

  • Western Blot (WB) analysis of various lysates using CACNA2D2 Polyclonal antibody (22145-1-AP)

    WB analysis using 22145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22145-1-AP (CACNA2D2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse brain tissue, SH-SY5Y cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22145-1-AP targets CACNA2D2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACNA2D2 fusion protein Ag17580 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, alpha 2/delta subunit 2
Calculated molecular weight 1145 aa, 129 kDa
Observed molecular weight100-112 kDa
GenBank accession numberBC152438
Gene symbol CACNA2D2
Gene ID (NCBI) 9254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CACNA2D2 (calcium voltage-gated channel auxiliary subunit alpha2delta 2), also known as CASVDD. It is expected to be located in cell membrane. The protein is predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level). Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain (PMID: 10766861). The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel (PMID: 15111129). The molecular weight of CACNA2D2 is 129 kDa, and it can recognize other isoforms, which can be cut into fragments.

Protocols

Product Specific Protocols
WB protocol for CACNA2D2 antibody 22145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols