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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CACNA2D4 Polyclonal antibody (26674-1-AP)

    WB analysis using 26674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26674-1-AP (CACNA2D4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CACNA2D4 Polyclonal antibody

CACNA2D4 Polyclonal Antibody for WB, ELISA
Cat No. 26674-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Voltage-gated calcium channel subunit alpha-2/delta-4, Voltage-dependent calcium channel subunit delta-4, Voltage-dependent calcium channel subunit alpha-2-4, Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26674-1-AP targets CACNA2D4 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACNA2D4 fusion protein Ag24464 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, alpha 2/delta subunit 4
Calculated molecular weight 1137 aa, 128 kDa
Observed molecular weight100 kDa
GenBank accession numberBC150186
Gene Symbol CACNA2D4
Gene ID (NCBI) 93589
RRIDAB_3669562
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3S7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcium voltage-gated channel auxiliary subunit alpha2delta 4 (CACNA2D4, also known as RCD4) is a member of the alpha-2/delta subunit family within the voltage-dependent calcium channel complex. The α2δ4 subunit of retinal voltage-gated calcium channels (Cav), is crucial for developing and mature exocytotic function of the photoreceptor ribbon synapse (PMID: 29875267). CACNA2D4 is localized to the retina, particularly in photoreceptors, where it is essential for organizing synaptic ribbons and setting Cav1.4 voltage sensitivity (PMID: 28262416). In humans, mutations in CACNA2D4 are associated with autosomal recessive cone dystrophy, a condition that affects the photoreceptor cells in the retina (PMID: 16877424).

Protocols

Product Specific Protocols
WB protocol for CACNA2D4 antibody 26674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols