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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CACNB3 Recombinant antibody (84762-3-RR)

    WB analysis using 84762-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84762-3-RR (CACNB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CACNB3 Recombinant antibody (84762-3-RR)

    WB analysis using 84762-3-RR

    Mouse brain tissue were subjected to SDS PAGE followed by western blot with 84762-3-RR (CACNB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84762-3-RR

    Affinity and Kinetic Characterization of 84762-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84762-3-RR against Human CACNB3 were performed. The affinity constant is 0.241 nM.

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CACNB3 Recombinant antibody

CACNB3 Recombinant Antibody for WB, ELISA
Cat No. 84762-3-RR
Clone No.242088G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CACNLB3, CACNB 3, CAB3, 242088G1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, mouse brain tissue, HeLa cells, SH-SY5Y cells, SKOV-3 cells, SW480 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84762-3-RR targets CACNB3 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CACNB3 fusion protein Ag35651 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, beta 3 subunit
Calculated molecular weight55kDa，484aa
Observed molecular weight58 kDa
GenBank accession numberNM_000725.3
Gene Symbol CACNB3
Gene ID (NCBI) 784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54284
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CACNB3, also known as CACNLB3, is a voltage-gated Ca2+ channel subunit. It has 5 isoforms and may play a role in the regulation of transcription factors and calcium transport.

Protocols

Product Specific Protocols
WB protocol for CACNB3 antibody 84762-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols