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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CACNB4 Polyclonal antibody

CACNB4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17770-1-AP

Synonyms

CAB4, CACNB4, CACNLB4, EA5, EJM

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CACNB4 Polyclonal antibody (17770-1-AP)

    WB analysis using 17770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17770-1-AP (CACNB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CACNB4 Polyclonal antibody (17770-1-AP)

    WB analysis of rat brain using 17770-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17770-1-AP (CACNB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CACNB4 Polyclonal antibody (17770-1-AP)

    IHC staining of mouse brain using 17770-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17770-1-AP (CACNB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CACNB4 Polyclonal antibody (17770-1-AP)

    IHC staining of mouse brain using 17770-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17770-1-AP (CACNB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 17770-1-AP is CACNB4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACNB4 fusion protein Ag12178 相同性解析による交差性が予測される生物種
Full Name calcium channel, voltage-dependent, beta 4 subunit
Calculated molecular weight 520 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC075049
Gene symbol CACNB4
Gene ID (NCBI) 785
RRIDAB_2878438
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CACNB4 antibody 17770-1-APDownload protocol
IHC protocol for CACNB4 antibody 17770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p.

Authors - Cui Ma
View Article