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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CACTIN Polyclonal antibody (32066-1-AP)

    WB analysis using 32066-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32066-1-AP (CACTIN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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CACTIN Polyclonal antibody

CACTIN Polyclonal Antibody for WB, ELISA
Cat No. 32066-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Splicing factor Cactin, Renal carcinoma antigen NY-REN-24

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32066-1-AP targets CACTIN in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CACTIN fusion protein Ag35645 相同性解析による交差性が予測される生物種
Full Name chromosome 19 open reading frame 29
Calculated molecular weight 108 kDa, 89 kDa
Observed molecular weight120 kDa
GenBank accession numberNM_021231
Gene Symbol C19orf29
Gene ID (NCBI) 58509
RRIDAB_3670183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8WUQ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CACTIN plays a role in pre-mRNA splicing by facilitating excision of a subset of introns (PubMed:28062851).CACTIN was reported to be involved in the regulation of innate immune response (PubMed:20829348). CACTIN acts as a negative regulator of Toll-like receptor, interferon-regulatory factor (IRF) and canonical NF-kappa-B signaling pathways (PubMed:20829348, PubMed:26363554). CACTIN contributes to the regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous pro-inflammatory stimuli (PubMed:20829348, PubMed:26363554). CACTIN has two isforms (89kDa and 108 kDa).

Protocols

Product Specific Protocols
WB protocol for CACTIN antibody 32066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols