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CAD Polyclonal antibody
CAD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16617-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human liver cancer tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 8 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
16617-1-AP targets CAD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CAD fusion protein Ag9912 相同性解析による交差性が予測される生物種 |
Full Name | carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase |
Calculated molecular weight | 2225 aa, 243 kDa |
Observed molecular weight | 240 kDa |
GenBank accession number | BC014178 |
Gene symbol | CAD |
Gene ID (NCBI) | 790 |
RRID | AB_2878289 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides (PMID:25422319). CAD initiates and controls the flflux through the pathway and is regulated by allosteric effectors and phosphorylations through different signaling cascades (PMID:12438317). Moreover, CAD is found upregulated in tumors, while mutations that compromise its function are the cause of a severe infantile epileptic disease (PMID:36677714).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CAD antibody 16617-1-AP | Download protocol |
IHC protocol for CAD antibody 16617-1-AP | Download protocol |
IF protocol for CAD antibody 16617-1-AP | Download protocol |
IP protocol for CAD antibody 16617-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate | ||
Theranostics Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription. | ||
PLoS Biol Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway. | ||
Front Cell Dev Biol Identification of Key Genes Related With Aspartic Acid Metabolism and Corresponding Protein Expression in Human Colon Cancer With Postoperative Prognosis and the Underlying Molecular Pathways Prediction. | ||
Cancer Cell Int p53 activation enhances the sensitivity of non-small cell lung cancer to the combination of SH003 and docetaxel by inhibiting de novo pyrimidine synthesis |