CADM1 Polyclonal antibody
CADM1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 14335-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, A549 cells, human placenta tissue, mouse lung tissue |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
14335-1-AP targets CADM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CADM1 fusion protein Ag5621 相同性解析による交差性が予測される生物種 |
| Full Name | cell adhesion molecule 1 |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 49-60 kDa |
| GenBank accession number | BC047021 |
| Gene symbol | CADM1 |
| Gene ID (NCBI) | 23705 |
| RRID | AB_2243801 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CADM1 (cell adhesion molecule 1), also referred to as SgIGSF, Necl-2 or TSLC-1, is a cell adhesion molecule belonging to the immunoglobulin superfamily. It can mediate homophilic and heterophilic cell-cell adhesion. CADM1 is expressed by a variety of cells including neurons, mast cells, spermatogonia, pancreatic islet cells, epithelial cells such as lung alveolar cells and biliary epithelial cells. CADM1 is involved in carcinoma pathogenesis and the expression of CADM1 is frequently down-regulated in various tumors derived from epithelial cells. It acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. (PMID: 18332875; 17326163; 20340131)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CADM1 antibody 14335-1-AP | Download protocol |
| IHC protocol for CADM1 antibody 14335-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS One Necl-4/SynCAM-4 is expressed in myelinating oligodendrocytes but not required for axonal myelination. | ||
Onco Targets Ther miR-424-5p Promotes Proliferation, Migration and Invasion of Laryngeal Squamous Cell Carcinoma. | ||
Oncol Lett miR-214-3p promotes the proliferation, migration and invasion of osteosarcoma cells by targeting CADM1. | ||
BMC Cancer CADM1 impairs the effect of miR-1246 on promoting cell cycle progression in chemo-resistant leukemia cells | ||
Fish Shellfish Immunol Mucous cell histopathology and label-free quantitative proteomic analysis of skin mucus in fat greenling (Hexagrammos otakii) infected with Vibrio harveyi | ||
Adv Healthc Mater Nanointegrative Glycoengineering-Activated Necroptosis of Triple Negative Breast Cancer Stem Cells Enables Self-Amplifiable Immunotherapy for Systemic Tumor Rejection |
