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Calbindin-D28k Polyclonal antibody
Calbindin-D28k Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, IF-Fro, IP, ELISA
Conjugate
Unconjugated
Cat no : 14479-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human cerebellum tissue, hippocampus, human brain tissue, mouse cerebellum tissue, rat cerebellum tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue, human kidney tissue |
| Positive IF-Fro detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 8 publications below |
| IF | See 18 publications below |
Product Information
14479-1-AP targets Calbindin-D28k in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Calbindin-D28k fusion protein Ag5861 相同性解析による交差性が予測される生物種 |
| Full Name | calbindin 1, 28kDa |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC006478 |
| Gene symbol | Calbindin |
| Gene ID (NCBI) | 793 |
| RRID | AB_2228318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Calbindin-D28k antibody 14479-1-AP | Download protocol |
| IHC protocol for Calbindin-D28k antibody 14479-1-AP | Download protocol |
| IF protocol for Calbindin-D28k antibody 14479-1-AP | Download protocol |
| IP protocol for Calbindin-D28k antibody 14479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice. | ||
Nat Neurosci Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage | ||
Cell Death Differ The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum. | ||
Mol Ther sEVsRVG selectively delivers antiviral siRNA to fetus brain, inhibits ZIKV infection and mitigates ZIKV-induced microcephaly in mouse model. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Georgiana (Verified Customer) (09-11-2023) | Antibody labelled cell bodies and dendrites very clearly
 |
FH Ramzi (Verified Customer) (06-14-2023) | The brain vibratome sections were treated with Acid-Alcohol before the blocking solution.
 |
FH Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
|
FH Carly (Verified Customer) (11-17-2020) | Tested using EDTA plasma on an antibody microarray
|
