Calretinin Polyclonal antibody

Name: Calretinin Polyclonal antibody
Brand: Proteintech
SKU: 12278-1-AP

Calretinin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12278-1-AP

Synonyms

29 kDa calbindin, CAB29, CAL2, CALB2, calbindin 2, Calretinin, CR

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 12278-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.
WB analysis of human ileum using 12278-1-AP
human ileum tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 12278-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12278-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 12278-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 12278-1-AP (Calretinin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12278-1-AP
IP result of anti-Calretinin (IP:12278-1-AP, 4ug; Detection:12278-1-AP 1:10000) with mouse brain tissue lysate 1280 ug.
IHC staining of human appendicitis using 12278-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 12278-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12278-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12278-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12278-1-AP (Calretinin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 12278-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 12278-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human cerebellum using 12278-1-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human cerebellum using 12278-1-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 12278-1-AP (Calretinin Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 12278-1-AP

Size/Concentration

ご購入について

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	fetal human brain tissue, human ileum tissue, human brain tissue, human colon tissue, mouse brain tissue, mouse colon tissue, rat brain tissue, rat colon tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human appendicitis tissue, mouse brain tissue, human brain tissue, human cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 3 publications below
IF	See 8 publications below

Product Information

12278-1-AP targets Calretinin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Calretinin fusion protein Ag2924 相同性解析による交差性が予測される生物種
Full Name	calbindin 2
Calculated molecular weight	29 kDa
Observed molecular weight	29 kDa
GenBank accession number	BC015484
Gene symbol	Calretinin
Gene ID (NCBI)	794
RRID	AB_2228338
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

Protocols

Product Specific Protocols
WB protocol for Calretinin antibody 12278-1-AP	Download protocol
IHC protocol for Calretinin antibody 12278-1-AP	Download protocol
IP protocol for Calretinin antibody 12278-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Rep Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment Authors - Atsuko Yonemura View Article
mouse	IF	Front Cell Dev Biol Stepwise Induction of Inner Ear Hair Cells From Mouse Embryonic Fibroblasts via Mesenchymal- to-Epithelial Transition and Formation of Otic Epithelial Cells. Authors - Qiong Yang View Article
human	IF	Stem Cells Int Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids Authors - Ke Ning View Article
human	IHC	Front Mol Biosci Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts. Authors - Hufei Wang View Article
mouse	IHC	Endocrinology Fetal Bisphenol-A Induced Changes in Murine Behavior and Brain Gene Expression Persist in Aged Offspring. Authors - Zhihao Wang View Article
mouse	IF	Invest Ophthalmol Vis Sci Primary Cilia in Amacrine Cells in Retinal Development. Authors - Ke Ning View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calretinin Polyclonal antibody

Calretinin Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 12278-1-AP

WB analysis of human ileum using 12278-1-AP

WB analysis of fetal human brain using 12278-1-AP

WB analysis of human brain using 12278-1-AP

WB analysis of human colon using 12278-1-AP

IP experiment of mouse brain using 12278-1-AP

IHC staining of human appendicitis using 12278-1-AP

IHC staining of human appendicitis using 12278-1-AP

IHC staining of mouse brain using 12278-1-AP

IHC staining of mouse brain using 12278-1-AP

IHC staining of human brain using 12278-1-AP

IHC staining of human brain using 12278-1-AP

IHC staining of human cerebellum using 12278-1-AP

IHC staining of human cerebellum using 12278-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment

Stepwise Induction of Inner Ear Hair Cells From Mouse Embryonic Fibroblasts via Mesenchymal- to-Epithelial Transition and Formation of Otic Epithelial Cells.

Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids

Tumor Microenvironment Heterogeneity-Based Score System Predicts Clinical Prognosis and Response to Immune Checkpoint Blockade in Multiple Colorectal Cancer Cohorts.

Fetal Bisphenol-A Induced Changes in Murine Behavior and Brain Gene Expression Persist in Aged Offspring.

Primary Cilia in Amacrine Cells in Retinal Development.