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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CALCA/CGRP Polyclonal antibody (22244-1-AP)

    WB analysis using 22244-1-AP

    TT cells were subjected to SDS PAGE followed by western blot with 22244-1-AP (CGRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using CALCA/CGRP Polyclonal antibody (22244-1-AP)

    WB analysis of Transfected HEK-293 using 22244-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22244-1-AP (CGRP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of TT cells using CALCA/CGRP Polyclonal antibody (22244-1-AP)

    IP experiment of TT using 22244-1-AP

    IP result of anti-CALCA/CGRP (IP:22244-1-AP, 4ug; Detection:22244-1-AP 1:600) with TT cells lysate 945 ug.

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CALCA/Calcitonin Polyclonal antibody

CALCA/Calcitonin Polyclonal Antibody for WB, IP, ELISA
Cat No. 22244-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

CALCA/CGRP, CALCA, Calcitonin, CALC1, Calcitonin gene-related peptide 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTT cells, Transfected HEK-293 cells
Positive IP detected inTT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22244-1-AP targets CALCA/Calcitonin in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALCA/Calcitonin fusion protein Ag17737 相同性解析による交差性が予測される生物種
Full Name Calcitonin
Calculated molecular weight 141 aa, 15 kDa
Observed molecular weight14 kDa, 5 kDa
GenBank accession numberBC093753
Gene Symbol CALCA
Gene ID (NCBI) 796
RRIDAB_3669393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.

Protocols

Product Specific Protocols
WB protocol for CALCA/Calcitonin antibody 22244-1-APDownload protocol
IP protocol for CALCA/Calcitonin antibody 22244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols