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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CALCA/CGRP Polyclonal antibody

CALCA/CGRP Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22244-1-AP

Synonyms

Alpha type CGRP, CALC1, CALCA, Calcitonin, CGRP, CGRP I, CGRP1, CT, KC, Procalcitonin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CALCA/CGRP Polyclonal antibody (22244-1-AP)

    WB analysis using 22244-1-AP

    TT cells were subjected to SDS PAGE followed by western blot with 22244-1-AP (CGRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using CALCA/CGRP Polyclonal antibody (22244-1-AP)

    WB analysis of Transfected HEK-293 using 22244-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22244-1-AP (CGRP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of TT cells using CALCA/CGRP Polyclonal antibody (22244-1-AP)

    IP experiment of TT using 22244-1-AP

    IP result of anti-CALCA/CGRP (IP:22244-1-AP, 4ug; Detection:22244-1-AP 1:600) with TT cells lysate 945 ug.

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Tested Applications

Positive WB detected inTT cells, Transfected HEK-293 cells
Positive IP detected inTT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22244-1-AP targets CALCA/CGRP in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALCA/CGRP fusion protein Ag17737 相同性解析による交差性が予測される生物種
Full Name calcitonin-related polypeptide alpha
Calculated molecular weight 141 aa, 15 kDa
Observed molecular weight14 kDa, 5 kDa
GenBank accession numberBC093753
Gene symbol CGRP
Gene ID (NCBI) 796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide, which is primarily localized to C and Aδ sensory fibers. These fibers display a wide innervation throughout the body, with extensive perivascular localization, and have a dual role in sensory (nociceptive) and efferent (effector) function. Discovered 30 years ago, it is produced as a consequence of alternative RNA processing of the calcitonin gene. CGRP has two major forms (α and β).

Protocols

Product Specific Protocols
WB protocol for CALCA/CGRP antibody 22244-1-APDownload protocol
IP protocol for CALCA/CGRP antibody 22244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols