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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CALCA/CGRP Recombinant antibody

CALCA/CGRP Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240753G2

Cat no : 83774-1-RR

Synonyms

Calcitonin, CALCA, CALC1, Alpha-type CGRP, Alpha type CGRP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of TT cells using CALCA/CGRP Recombinant antibody (83774-1-RR)

    IF Staining of TT using 83774-1-RR

    Immunofluorescent analysis of (4% PFA) fixed TT cells using Procalcitonin/CALCA antibody (83774-1-RR, Clone: 240753G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).(negative control: HeLa cells)

  • Flow cytometry (FC) experiment of TT cells using CALCA/CGRP Recombinant antibody (83774-1-RR)

    FC experiment of TT using 83774-1-RR

    1x10^6 TT cells were intracellularly stained with 0.25 ug Procalcitonin/CALCA Recombinant Antibody (83774-1-RR, Clone:240753G2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83774-1-RR

    Affinity and Kinetic Characterization of 83774-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83774-1-RR against Human CALCA/CGRP were performed. The affinity constant is 0.189 nM.

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Tested Applications

Positive IF/ICC detected inTT cells
Positive FC (Intra) detected inTT cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83774-1-RR is CALCA/CGRP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name calcitonin-related polypeptide alpha
Calculated molecular weight15kDa
GenBank accession numberNM_001741.3
Gene symbol CGRP
Gene ID (NCBI) 796
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide, which is primarily localized to C and Aδ sensory fibers. These fibers display a wide innervation throughout the body, with extensive perivascular localization, and have a dual role in sensory (nociceptive) and efferent (effector) function. Discovered 30 years ago, it is produced as a consequence of alternative RNA processing of the calcitonin gene. CGRP has two major forms (α and β).

Protocols

Product Specific Protocols
IF protocol for CALCA/CGRP antibody 83774-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols