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- KD/KO Validated
CALCOCO1 Polyclonal antibody
CALCOCO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 19843-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HT-1080 cells, HeLa cells, MCF-7 cells, Ramos cells, NIH/3T3 cells |
| Positive IHC detected in | human testis tissue, human kidney tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
19843-1-AP targets CALCOCO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CALCOCO1 fusion protein Ag13876 相同性解析による交差性が予測される生物種 |
| Full Name | calcium binding and coiled-coil domain 1 |
| Calculated molecular weight | 691 aa, 77 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC003177 |
| Gene symbol | CALCOCO1 |
| Gene ID (NCBI) | 57658 |
| RRID | AB_10637265 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CALCOCO1 antibody 19843-1-AP | Download protocol |
| IHC protocol for CALCOCO1 antibody 19843-1-AP | Download protocol |
| IF protocol for CALCOCO1 antibody 19843-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Autophagy regulates inflammatory programmed cell death via turnover of RHIM-domain proteins.
| ||
Cell Signal Fam83d modulates MAP kinase and AKT signaling and is induced during neurogenic skeletal muscle atrophy. | ||
Nat Commun Tumor-intrinsic expression of the autophagy gene Atg16l1 suppresses anti-tumor immunity in colorectal cancer |
