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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CALCOCO1 Recombinant antibody, PBS Only

CALCOCO1 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241127G7

Cat no : 84009-4-PBS

Synonyms

PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CALCOCO1 Recombinant antibody (84009-4-RR)

    WB analysis using 84009-4-RR (same clone as 84009-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84009-4-RR (CALCOCO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84009-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CALCOCO1 Recombinant antibody (84009-4-RR)

    IHC staining of mouse testis using 84009-4-RR (same clone as 84009-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84009-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using CALCOCO1 Recombinant antibody (84009-4-RR)

    IHC staining of rat testis using 84009-4-RR (same clone as 84009-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84009-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using CALCOCO1 Recombinant antibody (84009-4-RR)

    IHC staining of rat testis using 84009-4-RR (same clone as 84009-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84009-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CALCOCO1 Recombinant antibody (84009-4-RR)

    IHC staining of mouse testis using 84009-4-RR (same clone as 84009-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84009-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84009-4-RR

    Affinity and Kinetic Characterization of 84009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84009-4-RR against Human CALCOCO1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84009-4-PBS is CALCOCO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CALCOCO1 fusion protein Ag13876 相同性解析による交差性が予測される生物種
Full Name calcium binding and coiled-coil domain 1
Calculated molecular weight 691 aa, 77 kDa
Observed molecular weight100 kDa
GenBank accession numberBC003177
Gene symbol CALCOCO1
Gene ID (NCBI) 57658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.