Calmodulin Polyclonal antibody

Calmodulin Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10541-1-AP

Synonyms

Calmodulin 3, Calmodulin 1/2/3, CALML2, CALM3, CAM3


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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, HepG2 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NCCIT cells, rat skeletal muscle tissue
Positive IP detected inRamos cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10541-1-AP targets Calmodulin in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calmodulin fusion protein Ag0827 相同性解析による交差性が予測される生物種
Full Name calmodulin 3 (phosphorylase kinase, delta)
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC006182
Gene symbol Calmodulin 3
Gene ID (NCBI) 808
RRIDAB_2069442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calmodulin (CaM) is a Ca(2+)-binding protein that transduces Ca2+-mediated signals by binding to and regulating the activity of hundreds of enzymes and non-enzymatic proteins. It is highly conserved across species and involved in many biological processes, including vesicle release, cell proliferation, and apoptosis. This antibody can recognize CALM1, CALM2 and CALM3.

Protocols

Product Specific Protocols
WB protocol for Calmodulin antibody 10541-1-APDownload protocol
IHC protocol for Calmodulin antibody 10541-1-APDownload protocol
IP protocol for Calmodulin antibody 10541-1-APDownload protocol
FC protocol for Calmodulin antibody 10541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
human

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
humanIHC, WB

Cancer Cell Int

CALM1 promotes progression and dampens chemosensitivity to EGFR inhibitor in esophageal squamous cell carcinoma.

Authors - Tao Liu
IP

Neurochem Int

Alpha-synuclein overexpression increases phospho-protein phosphatase 2A levels via formation of calmodulin/Src complex.

Authors - W Yang
humanWB

Biochem Biophys Res Commun

The NMDAR GluN1-1a C-terminus binds to CaM and regulates synaptic function.

Authors - Liang Zhou
humanWB

J Cardiovasc Transl Res

TRPC1 Deficiency Impairs the Endothelial Progenitor Cell Function via Inhibition of Calmodulin/eNOS Pathway.

Authors - Lai-Ling Du