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KD/KO Validated

CALML4 Polyclonal antibody

Name: CALML4 Polyclonal antibody
Brand: Proteintech
SKU: 15894-1-AP

CALML4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15894-1-AP

Synonyms

CALML4, calmodulin like 4, Calmodulin like protein 4, NY BR 20

Validation Data Gallery

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AllWBIP

WB analysis using 15894-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15894-1-AP (CALML4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 15894-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 15894-1-AP (CALML4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 15894-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15894-1-AP (CALML4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of mouse thymus using 15894-1-AP
IP result of anti-CALML4 (IP:15894-1-AP, 4ug; Detection:15894-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

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Cat No. 15894-1-AP

Size/Concentration

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, mouse thymus tissue, human colon tissue, rat kidney tissue
Positive IP detected in	mouse thymus tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IF	See 1 publications below

Product Information

15894-1-AP targets CALML4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CALML4 fusion protein Ag8705 相同性解析による交差性が予測される生物種
Full Name	calmodulin-like 4
Calculated molecular weight	196 aa, 22 kDa
Observed molecular weight	22-25 kDa
GenBank accession number	BC009516
Gene symbol	CALML4
Gene ID (NCBI)	91860
RRID	AB_2069462
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CALML4 antibody 15894-1-AP	Download protocol
IP protocol for CALML4 antibody 15894-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	ACS Chem Neurosci Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine. Authors - Lian Liu View Article
human	WB,IF	J Biol Chem The small EF-hand protein CALML4 functions as a critical myosin light chain within the intermicrovillar adhesion complex. Authors - Myoung Soo Choi KD Validated View Article
human	WB	J Biol Chem Molecular Regulatory Mechanism of Human Myosin-7a Authors - Alexandra Holló View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CALML4 Polyclonal antibody

CALML4 Polyclonal Antibody for WB, IP, ELISA

Validation Data Gallery

WB analysis using 15894-1-AP

WB analysis of human colon using 15894-1-AP

WB analysis of mouse thymus using 15894-1-AP

IP experiment of mouse thymus using 15894-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Tetrahydropalmatine Regulates BDNF through TrkB/CAM Interaction to Alleviate the Neurotoxicity Induced by Methamphetamine.

The small EF-hand protein CALML4 functions as a critical myosin light chain within the intermicrovillar adhesion complex.

Molecular Regulatory Mechanism of Human Myosin-7a