Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat brain tissue, human brain tissue, mouse brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
11477-1-AP targets CALN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, monkey, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CALN1 fusion protein Ag2043 相同性解析による交差性が予測される生物種 |
| Full Name | calneuron 1 |
| Calculated molecular weight | 219 aa, 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC020200 |
| Gene Symbol | CALN1 |
| Gene ID (NCBI) | 83698 |
| RRID | AB_2228454 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXU9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CALN1 antibody 11477-1-AP | Download protocol |
| IHC protocol for CALN1 antibody 11477-1-AP | Download protocol |
| IP protocol for CALN1 antibody 11477-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids circEYA1 Functions as a Sponge of miR-582-3p to Suppress Cervical Adenocarcinoma Tumorigenesis via Upregulating CXCL14. | ||
Hypertension Calneuron 1 Increased Ca2+ in the Endoplasmic Reticulum and Aldosterone Production in Aldosterone-Producing Adenoma. | ||
J Biol Chem Post-translational membrane insertion of tail-anchored transmembrane EF-hand Ca2+ sensor calneurons requires the TRC40/Asna1 protein chaperone. | ||
J Cell Mol Med Identification of core genes in prefrontal cortex and hippocampus of Alzheimer's disease based on mRNA-miRNA network | ||
Biochem Biophys Res Commun Exosomal miR-675 from metastatic osteosarcoma promotes cell migration and invasion by targeting CALN1. |