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calreticulin Polyclonal antibody

Name: calreticulin Polyclonal antibody
Brand: Proteintech
SKU: 10292-1-AP

calreticulin Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10292-1-AP

Synonyms

CALR, Calregulin

Validation Data Gallery

Filter:

AllWBIHCFC (Intra)

WB analysis using 10292-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10292-1-AP
WB result of calreticulin antibody (10292-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-calreticulin transfected HeLa cells.
WB analysis using 10292-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 10292-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 10292-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 10292-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 10292-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10292-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 10292-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 10292-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 10292-1-AP (calreticulin Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human thyroid using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 10292-1-AP (calreticulin Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human thyroid using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 10292-1-AP (calreticulin Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse colon using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10292-1-AP (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10292-1-AP (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10292-1-AP (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10292-1-AP (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10292-1-AP (calreticulin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10292-1-AP (calreticulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded normal colon slide using 10292-1-AP (calreticulin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10292-1-AP (calreticulin Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skeletal muscle using 10292-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10292-1-AP (calreticulin Antibody) at dilution of 1:200 (under 40x lens).
FC experiment of Jurkat using 10292-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human calreticulin (10292-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 10292-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HL-60 cells, A549 cells, mouse brain tissue, COLO 320 cells, NIH/3T3 cells, human skeletal muscle tissue, rat brain tissue, HeLa cells, HepG2 cells, MCF-7 cells, SH-SY5Y cells
Positive IHC detected in	human thyroid tissue, human normal colon, human skeletal muscle tissue, human thyroid cancer tissue, mouse colon tissue, mouse lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 45 publications below
IHC	See 13 publications below
FC	See 3 publications below
CoIP	See 1 publications below

Product Information

10292-1-AP targets calreticulin in WB, IHC, FC (Intra), CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	calreticulin fusion protein Ag0325 相同性解析による交差性が予測される生物種
Full Name	calreticulin
Calculated molecular weight	60 kDa
Observed molecular weight	55 kDa
GenBank accession number	BC002500
Gene symbol	Calreticulin
Gene ID (NCBI)	811
RRID	AB_2069615
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CALR,also named as grp60, ERp60, HACBP, CRP55, CRTC and Calregulin, belongs to the calreticulin family. It is a molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. CALR is a ER marker. It interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. CALR interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. The MW of CALR migrates aberrantly at 55 kDa by SDS-PAGE. Some study provided that it's a new possibility for CRT-mediated tumor immune prevention and treatment.

Protocols

Product Specific Protocols
WB protocol for calreticulin antibody 10292-1-AP	Download protocol
IHC protocol for calreticulin antibody 10292-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse		ACS Nano ER-Targeting PDT Converts Tumors into In Situ Therapeutic Tumor Vaccines. Authors - Xu Liu View Article
mouse	WB	ACS Nano Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy Authors - Qiqi Zhang View Article
human	WB	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
mouse	WB	Acta Pharm Sin B Antitumor synergism between PAK4 silencing and immunogenic phototherapy of engineered extracellular vesicles Authors - Mei Lu View Article
human	WB	Acta Pharm Sin B SIRT1 activation synergizes with FXR agonism in hepatoprotection via governing nucleocytoplasmic shuttling and degradation of FXR Authors - Shuang Cui View Article
mouse	Cell treatment	Small Methods Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy Authors - Shilin Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

calreticulin Polyclonal antibody

calreticulin Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 10292-1-AP

WB analysis of HeLa using 10292-1-AP

WB analysis using 10292-1-AP

WB analysis using 10292-1-AP

WB analysis using 10292-1-AP

WB analysis of human skeletal muscle using 10292-1-AP

WB analysis of mouse brain using 10292-1-AP

WB analysis of NIH/3T3 using 10292-1-AP

WB analysis of COLO 320 using 10292-1-AP

WB analysis of rat brain using 10292-1-AP

IHC staining of human thyroid using 10292-1-AP

IHC staining of human thyroid using 10292-1-AP

IHC staining of mouse colon using 10292-1-AP

IHC staining of mouse colon using 10292-1-AP

IHC staining of mouse lung using 10292-1-AP

IHC staining of mouse lung using 10292-1-AP

IHC staining of human thyroid cancer using 10292-1-AP

IHC staining of human thyroid cancer using 10292-1-AP

IHC staining of human normal colon using 10292-1-AP

IHC staining of human skeletal muscle using 10292-1-AP

IHC staining of human skeletal muscle using 10292-1-AP

FC experiment of Jurkat using 10292-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ER-Targeting PDT Converts Tumors into In Situ Therapeutic Tumor Vaccines.

Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Antitumor synergism between PAK4 silencing and immunogenic phototherapy of engineered extracellular vesicles

SIRT1 activation synergizes with FXR agonism in hepatoprotection via governing nucleocytoplasmic shuttling and degradation of FXR

Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy

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