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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CALR3 Polyclonal antibody

CALR3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17064-1-AP

Synonyms

CALR3, Calreticulin 2, calreticulin 3, Calreticulin-3, Calsperin, CRT2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using CALR3 Polyclonal antibody (17064-1-AP)

    WB analysis of mouse testis using 17064-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17064-1-AP (CALR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CALR3 Polyclonal antibody (17064-1-AP)

    IHC staining of human testis using 17064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17064-1-AP (CALR3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CALR3 Polyclonal antibody (17064-1-AP)

    IHC staining of human testis using 17064-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17064-1-AP (CALR3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17064-1-AP targets CALR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALR3 fusion protein Ag10847 相同性解析による交差性が予測される生物種
Full Name calreticulin 3
Calculated molecular weight 384 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC014595
Gene symbol CALR3
Gene ID (NCBI) 125972
RRIDAB_2243959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CALR3 antibody 17064-1-APDownload protocol
IHC protocol for CALR3 antibody 17064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols