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KD/KO Validated

Calumenin Polyclonal antibody

Name: Calumenin Polyclonal antibody
Brand: Proteintech
SKU: 17804-1-AP

Calumenin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17804-1-AP

Synonyms

CALU, Calumenin, Crocalbin, IEF SSP 9302

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HEK-293 using 17804-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 17804-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17804-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17804-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17804-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17804-1-AP (Calumenin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 17804-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 17804-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17804-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 17804-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17804-1-AP (Calumenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 17804-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (17804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 17804-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, human placenta tissue, K-562 cells, mouse kidney tissue
Positive IHC detected in	human placenta tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IF	See 1 publications below

Product Information

17804-1-AP targets Calumenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Calumenin fusion protein Ag12073 相同性解析による交差性が予測される生物種
Full Name	calumenin
Calculated molecular weight	315 aa, 37 kDa
Observed molecular weight	47 kDa, 57 kDa
GenBank accession number	BC013383
Gene symbol	Calumenin
Gene ID (NCBI)	813
RRID	AB_2069964
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Calumenin antibody 17804-1-AP	Download protocol
IHC protocol for Calumenin antibody 17804-1-AP	Download protocol
IF protocol for Calumenin antibody 17804-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
human	WB	Oncogene Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis. Authors - Q Wang KD Validated View Article
human	IF	Cancer Med Five EMT-related genes signature predicts overall survival and immune environment in microsatellite instability-high gastric cancer. Authors - Mili Zhang View Article
pig	WB	Cell Tissue Res Hormone-regulated PKA activity in porcine oviductal epithelial cells. Authors - Juan Manuel Teijeiro View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Calumenin Polyclonal antibody

Calumenin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 17804-1-AP

WB analysis of human placenta using 17804-1-AP

WB analysis of K-562 using 17804-1-AP

WB analysis of HepG2 using 17804-1-AP

WB analysis of mouse kidney using 17804-1-AP

IHC staining of human placenta using 17804-1-AP

IHC staining of human placenta using 17804-1-AP

IHC staining of mouse brain using 17804-1-AP

IHC staining of mouse brain using 17804-1-AP

IF Staining of HeLa using 17804-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis.

Five EMT-related genes signature predicts overall survival and immune environment in microsatellite instability-high gastric cancer.

Hormone-regulated PKA activity in porcine oviductal epithelial cells.