Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 24312-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24312-1-AP (CAMK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24312-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24312-1-AP (CAMK1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
IP experiment of HEK-293 using 24312-1-AP
IP result of anti-CAMK1 (IP:24312-1-AP, 4ug; Detection:24312-1-AP 1:300) with HEK-293 cells lysate 3400ug.
IP experiment of HEK-293 using 24312-1-AP
IP result of anti-CAMK1 (IP:24312-1-AP, 4ug; Detection:24312-1-AP 1:500) with HEK-293 cells lysate 2960 ug.
IHC staining of human colon cancer using 24312-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IHC staining of human colon cancer using 24312-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IHC staining of human breast cancer using 24312-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 24312-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 24312-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 24312-1-AP (CAMK1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

CAMK1 Polyclonal antibody

CAMK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 24312-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CaM KI, CaM kinase I, CaM kinase I alpha, CAMK1, CAMKI, CaMKI alpha

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

24312-1-AP targets CAMK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CAMK1 fusion protein Ag19449 相同性解析による交差性が予測される生物種
Full Name	calcium/calmodulin-dependent protein kinase I
Calculated molecular weight	370 aa, 41 kDa
Observed molecular weight	41 kDa
GenBank accession number	BC106755
Gene Symbol	CAMK1
Gene ID (NCBI)	8536
RRID	AB_2879489
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14012
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CAMK1 antibody 24312-1-AP	Download protocol
IHC protocol for CAMK1 antibody 24312-1-AP	Download protocol
IF protocol for CAMK1 antibody 24312-1-AP	Download protocol
IP protocol for CAMK1 antibody 24312-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Biomed Res Int Effect of Lentivirus-Mediated miR-499a-3p on Human Umbilical Vein Endothelial Cells. Authors - Huilei Zheng View Article
mouse	IHC	Biochim Biophys Acta Mol Basis Dis IGF2BP3-stabilized CAMK1 regulates the mitochondrial dynamics of renal tubule to alleviate diabetic nephropathy Authors - Du Yuan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 24312-1-AP

WB analysis of HEK-293 using 24312-1-AP

IP experiment of HEK-293 using 24312-1-AP