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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAMK1 Polyclonal antibody

CAMK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24312-1-AP

Synonyms

CaM KI, CaM kinase I, CaM kinase I alpha, CAMK1, CAMKI, CaMKI alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CAMK1 Polyclonal antibody (24312-1-AP)

    WB analysis using 24312-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24312-1-AP (CAMK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CAMK1 Polyclonal antibody (24312-1-AP)

    WB analysis of HEK-293 using 24312-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24312-1-AP (CAMK1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CAMK1 Polyclonal antibody (24312-1-AP)

    IP experiment of HEK-293 using 24312-1-AP

    IP result of anti-CAMK1 (IP:24312-1-AP, 4ug; Detection:24312-1-AP 1:300) with HEK-293 cells lysate 3400ug.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CAMK1 Polyclonal antibody (24312-1-AP)

    IP experiment of HEK-293 using 24312-1-AP

    IP result of anti-CAMK1 (IP:24312-1-AP, 4ug; Detection:24312-1-AP 1:500) with HEK-293 cells lysate 2960 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CAMK1 Polyclonal antibody (24312-1-AP)

    IHC staining of human colon cancer using 24312-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CAMK1 Polyclonal antibody (24312-1-AP)

    IHC staining of human colon cancer using 24312-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CAMK1 Polyclonal antibody (24312-1-AP)

    IHC staining of human breast cancer using 24312-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CAMK1 Polyclonal antibody (24312-1-AP)

    IHC staining of human breast cancer using 24312-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24312-1-AP (CAMK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CAMK1 Polyclonal antibody (24312-1-AP)

    IF Staining of HEK-293 using 24312-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 24312-1-AP (CAMK1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24312-1-AP is CAMK1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAMK1 fusion protein Ag19449 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase I
Calculated molecular weight 370 aa, 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC106755
Gene symbol CAMK1
Gene ID (NCBI) 8536
RRIDAB_2879489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAMK1 antibody 24312-1-APDownload protocol
IHC protocol for CAMK1 antibody 24312-1-APDownload protocol
IF protocol for CAMK1 antibody 24312-1-APDownload protocol
IP protocol for CAMK1 antibody 24312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Res Int

Effect of Lentivirus-Mediated miR-499a-3p on Human Umbilical Vein Endothelial Cells.

Authors - Huilei Zheng
View Article
mouseIHC

Biochim Biophys Acta Mol Basis Dis

IGF2BP3-stabilized CAMK1 regulates the mitochondrial dynamics of renal tubule to alleviate diabetic nephropathy

Authors - Du Yuan
View Article