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  • Featured Product
  • KD/KO Validated

CAMK1D Polyclonal antibody

CAMK1D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13613-1-AP

Synonyms

CaM K1, CaM KI delta, CaM kinase I delta, CaM kinase ID, CAMK1D, CaMKI delta, CaMKI like protein kinase, CaMKID, CKLiK

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CAMK1D Polyclonal antibody (13613-1-AP)

    WB analysis of HeLa using 13613-1-AP

    WB result of CAMK1D antibody (13613-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMK1D transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using CAMK1D Polyclonal antibody (13613-1-AP)

    WB analysis using 13613-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    WB analysis of mouse pancreas using 13613-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    WB analysis of mouse thymus using 13613-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of mouse brain using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of mouse brain using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of mouse brain using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of mouse brain using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of human pancreas using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CAMK1D Polyclonal antibody (13613-1-AP)

    IHC staining of human pancreas using 13613-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse pancreas tissue, HeLa cells, mouse thymus tissue, rat pancreas tissue
Positive IHC detected inmouse brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13613-1-AP targets CAMK1D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAMK1D fusion protein Ag4517 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase ID
Calculated molecular weight 357 aa, 40 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC035745
Gene symbol CAMK1D
Gene ID (NCBI) 57118
RRIDAB_2228498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAMK1D antibody 13613-1-APDownload protocol
IHC protocol for CAMK1D antibody 13613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols