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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAMK2 Monoclonal antibody, PBS Only

CAMK2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E3B3

Cat no : 66843-1-PBS

Synonyms

CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMK2B, CAMK2D, CAMK2G, CAMKA, KIAA0968

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    WB analysis using 66843-1-Ig (same clone as 66843-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    WB analysis of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig cerebellum tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    WB analysis of pig cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    WB analysis of rat cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    WB analysis of mouse cerebellum using 66843-1-Ig (same clone as 66843-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    WB analysis of pig brain using 66843-1-Ig (same clone as 66843-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    WB analysis of rat brain using 66843-1-Ig (same clone as 66843-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66843-1-Ig (CAMK2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    IHC staining of mouse brain using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    IHC staining of human renal cell carcinoma using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    IHC staining of human renal cell carcinoma using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66843-1-Ig (CAMK2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CaMKII alpha Monoclonal antibody (66843-1-Ig)

    IF Staining of SH-SY5Y using 66843-1-Ig (same clone as 66843-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CAMK2 antibody (66843-1-Ig, Clone: 1E3B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66843-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66843-1-PBS targets CAMK2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAMK2 fusion protein Ag4736 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase II alpha
Calculated molecular weight 478 aa, 54 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberBC040457
Gene symbol CAMK2A
Gene ID (NCBI) 815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of CAMK2 family.