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KD/KO Validated

CaMKII Beta Polyclonal antibody

Name: CaMKII Beta Polyclonal antibody
Brand: Proteintech
SKU: 11533-1-AP

CaMKII Beta Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11533-1-AP

Synonyms

CaM kinase II subunit beta, CAM2, CaMK II subunit beta, CAMK2, CAMK2B, CAMKB, CaMKII beta

Validation Data Gallery

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AllWBIPIHC

WB analysis using 11533-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11533-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 11533-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11533-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11533-1-AP (CaMKII beta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11533-1-AP
IP result of anti-CaMKII Beta (IP:11533-1-AP, 4ug; Detection:11533-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
IHC staining of human gliomas using 11533-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 11533-1-AP (CaMKII beta antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 11533-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11533-1-AP (CaMKII beta antibody at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 11533-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11533-1-AP (CaMKII beta antibody at dilution of 1:50 (under 40x lens).

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Cat No. 11533-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, SH-SY5Y cells, human brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human gliomas tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

11533-1-AP targets CaMKII Beta in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, zebra finches, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CaMKII Beta fusion protein Ag2119 相同性解析による交差性が予測される生物種
Full Name	calcium/calmodulin-dependent protein kinase II beta
Calculated molecular weight	503 aa, 56 kDa
Observed molecular weight	50-70 kDa
GenBank accession number	BC019070
Gene symbol	CaMKII beta
Gene ID (NCBI)	816
RRID	AB_2275067
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta ), also named CAM2, CAMK2, and CAMKB, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2B is a member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. It plays a distinct role in the induction of anergy in T lymphocytes, by differential regulation of IL10 and IL2 gene transcription suggesting MEF2A as a molecular target that can integrate different calcium signals(PMID:22578382). This protein has 8 isoforms produced by alternative splicing with a molecular weight between 50 kDa and 73 kDa. This antibody may have cross-reaction with CAMK2A/D/G due to the high homology.

Protocols

Product Specific Protocols
WB protocol for CaMKII Beta antibody 11533-1-AP	Download protocol
IHC protocol for CaMKII Beta antibody 11533-1-AP	Download protocol
IP protocol for CaMKII Beta antibody 11533-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Aging (Albany NY) Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway. Authors - Ting-Ting Chen View Article
mouse	WB	Sci Total Environ Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons. Authors - Huanliang Liu View Article
human	WB,IHC	Front Oncol Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma. Authors - Qingan Jia KD Validated View Article
human	IP	Front Cell Neurosci Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis. Authors - Shuchao Wang View Article
mouse	WB	PLoS One Adaptive Gene Regulation in the Striatum of RGS9-Deficient Mice. Authors - Kathy Busse View Article
rat	WB,IF	Neural Plast Ketamine Induces Lasting Antidepressant Effects by Modulating the NMDAR/CaMKII-Mediated Synaptic Plasticity of the Hippocampal Dentate Gyrus in Depressive Stroke Model. Authors - Idriss Ali Abdoulaye View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CaMKII Beta Polyclonal antibody

CaMKII Beta Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis using 11533-1-AP

WB analysis of human brain using 11533-1-AP

WB analysis of SH-SY5Y using 11533-1-AP

WB analysis of human brain using 11533-1-AP

IP experiment of mouse brain using 11533-1-AP

IHC staining of human gliomas using 11533-1-AP

IHC staining of human brain using 11533-1-AP

IHC staining of human brain using 11533-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Gastrodin ameliorates learning and memory impairment in rats with vascular dementia by promoting autophagy flux via inhibition of the Ca2+/CaMKII signal pathway.

Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons.

Anti-Tumor Role of CAMK2B in Remodeling the Stromal Microenvironment and Inhibiting Proliferation in Papillary Renal Cell Carcinoma.

Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis.

Adaptive Gene Regulation in the Striatum of RGS9-Deficient Mice.

Ketamine Induces Lasting Antidepressant Effects by Modulating the NMDAR/CaMKII-Mediated Synaptic Plasticity of the Hippocampal Dentate Gyrus in Depressive Stroke Model.