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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAMK2D Recombinant antibody, PBS Only

CAMK2D Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1M10

Cat no : 81014-1-PBS

Synonyms

CaMKII delta, EC:2.7.11.17, CaMK-II subunit delta, CaM kinase II subunit delta, Calcium/calmodulin-dependent protein kinase type II subunit delta

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CAMK2D Recombinant antibody (81014-1-RR)

    WB analysis using 81014-1-RR (same clone as 81014-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81014-1-RR (CAMK2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CAMK2D Recombinant antibody (81014-1-RR)

    IHC staining of mouse brain using 81014-1-RR (same clone as 81014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81014-1-RR (CAMK2D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81014-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81014-1-RR

    Affinity and Kinetic Characterization of 81014-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81014-1-RR against Human CAMK2D were performed. The affinity constant is 81.6 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81014-1-PBS targets CAMK2D in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CAMK2D fusion protein Ag31276 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase II delta
Calculated molecular weight 59 kDa
Observed molecular weight54-59 kDa
GenBank accession numberBC032784
Gene symbol CAMK2D
Gene ID (NCBI) 817
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CaMKII delta, also named as CAMKD, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It may also recognize CAMK2A due to the high homology.