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CaMKII delta-Specific Polyclonal antibody

CaMKII delta-Specific Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 20667-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

CaMKII delta, CAMK2D, EC:2.7.11.17, CaMK-II subunit delta, CaM kinase II subunit delta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, mouse heart tissue, human skeletal muscle tissue, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20667-1-AP targets CaMKII delta-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase II delta
Calculated molecular weight 59 kDa
Observed molecular weight 55 kDa
GenBank accession numberNM_001221
Gene Symbol CAMK2D
Gene ID (NCBI) 817
RRIDAB_10695498
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13557
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CaMKII delta, also named as CAMKD and CaMKII delta, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It can recognize most isoforms the gene encodes, from 50-72kd. It can recognize the isoform 2,3,4,9,11 of CAMK2D.

Protocols

Product Specific Protocols
WB protocol for CaMKII delta-Specific antibody 20667-1-APDownload protocol
IHC protocol for CaMKII delta-Specific antibody 20667-1-APDownload protocol
IP protocol for CaMKII delta-Specific antibody 20667-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma

Authors - You Heng Chuah
humanWB

Mol Ther Nucleic Acids

circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p.

Authors - Cui Ma
mouseIHC

Cell Death Dis

miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2.

Authors - Xudong Zhang
humanWB

Reprod Biol Endocrinol

In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein

Authors - Jiaqi Tang
ratWB

J Pain Res

Proteomic Analysis of Emodin Treatment in Neuropathic Pain Reveals Dysfunction of the Calcium Signaling Pathway.

Authors - Peng Chen
humanWB

Oncol Lett

PMID: 30127991

Authors - Xiaoli Xu
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