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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAMKK1 Polyclonal antibody

CAMKK1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13624-1-AP

Synonyms

CaM KK 1, CaM kinase kinase alpha, CaM kinase kinase 1, CaM kinase IV kinase, Calcium/calmodulin-dependent protein kinase kinase alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CAMKK1 Polyclonal antibody (13624-1-AP)

    WB analysis of Jurkat using 13624-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using CAMKK1 Polyclonal antibody (13624-1-AP)

    IP experiment of Jurkat using 13624-1-AP

    IP result of anti-CAMKK1 (IP:13624-1-AP, 4ug; Detection:13624-1-AP 1:1000) with Jurkat cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of human stomach using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CAMKK1 Polyclonal antibody (13624-1-AP)

    IHC staining of human stomach using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13624-1-AP targets CAMKK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAMKK1 fusion protein Ag4534 相同性解析による交差性が予測される生物種
Full Name calcium/calmodulin-dependent protein kinase kinase 1, alpha
Calculated molecular weight 520 aa, 57 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC031647
Gene symbol CAMKK1
Gene ID (NCBI) 84254
RRIDAB_2068403
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calcium/calmodulin-dependent protein kinase kinase 1 catalyzes the phosphorylation and activation of Ca(2+)/calmodulin kinase (CaMK) as an upstream component of the CaMK cascade that has been implicated in neuronal gene transcription, synaptic plasticity, and long-term memory consolidation .CAMKK1 has two isoforms of 56kda and 58kda and involved in regulating cell apoptosis, promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death .Researches showed that Camkk1 plays a selective role in contextual fear memory.This 66-68kda protein can be phosphorylated(probably antophosphorylation)(PMID:7961813).This antibody recognize the N-terminal of CAMKK1 .

Protocols

Product Specific Protocols
WB protocol for CAMKK1 antibody 13624-1-APDownload protocol
IHC protocol for CAMKK1 antibody 13624-1-APDownload protocol
IP protocol for CAMKK1 antibody 13624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols