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KD/KO Validated

CAMKK2 Polyclonal antibody

Name: CAMKK2 Polyclonal antibody
Brand: Proteintech
SKU: 11549-1-AP

CAMKK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11549-1-AP

Synonyms

CaM KK beta, CaM KK 2, CaM kinase kinase beta, CaM kinase kinase 2, Calcium/calmodulin-dependent protein kinase kinase beta

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of Jurkat using 11549-1-AP
WB result of CAMKK2 antibody (11549-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMKK2 transfected Jurkat cells.
WB analysis using 11549-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 11549-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11549-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 11549-1-AP
IP result of anti-CAMKK2 (IP:11549-1-AP, 4ug; Detection:11549-1-AP 1:500) with PC-3 cells lysate 960 ug.
IHC staining of human prostate cancer using 11549-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human prostate cancer using 11549-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 40x lens).
IF Staining of PC-3 using 11549-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CAMKK2 antibody (11549-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11549-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, fetal human brain tissue, HeLa cells, PC-3 cells, RAW 264.7 cells
Positive IP detected in	PC-3 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 41 publications below
IHC	See 3 publications below
IF	See 6 publications below

Product Information

11549-1-AP targets CAMKK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CAMKK2 fusion protein Ag2093 相同性解析による交差性が予測される生物種
Full Name	calcium/calmodulin-dependent protein kinase kinase 2, beta
Calculated molecular weight	588 aa, 60 kDa
Observed molecular weight	60-70 kDa
GenBank accession number	BC026060
Gene symbol	CAMKK2
Gene ID (NCBI)	10645
RRID	AB_2259441
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CAMKK2 antibody 11549-1-AP	Download protocol
IHC protocol for CAMKK2 antibody 11549-1-AP	Download protocol
IF protocol for CAMKK2 antibody 11549-1-AP	Download protocol
IP protocol for CAMKK2 antibody 11549-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK. Authors - Chen-Song Zhang View Article
human	WB	Cancer Discov Macropinocytosis in Cancer-Associated Fibroblasts Is Dependent on CaMKK2/ARHGEF2 Signaling and Functions to Support Tumor and Stromal Cell Fitness. Authors - Yijuan Zhang KD Validated View Article
human	WB	Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress. Authors - Qidong Li KD Validated View Article
human	WB	Nat Commun Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents. Authors - Ziwen Wang View Article
human	WB	EMBO J CARS senses cysteine deprivation to activate AMPK for cell survival. Authors - Mengqiu Yuan KD Validated View Article
mouse	WB,IF	Autophagy m6A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells. Authors - Yabing Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAMKK2 Polyclonal antibody

CAMKK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 11549-1-AP

WB analysis using 11549-1-AP

WB analysis of fetal human brain using 11549-1-AP

WB analysis of HeLa using 11549-1-AP

IP experiment of PC-3 using 11549-1-AP

IHC staining of human prostate cancer using 11549-1-AP

IHC staining of human prostate cancer using 11549-1-AP

IF Staining of PC-3 using 11549-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.

Macropinocytosis in Cancer-Associated Fibroblasts Is Dependent on CaMKK2/ARHGEF2 Signaling and Functions to Support Tumor and Stromal Cell Fitness.

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.

CARS senses cysteine deprivation to activate AMPK for cell survival.

m6A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells.