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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CAMLG Polyclonal antibody

CAMLG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23327-1-AP

Synonyms

calcium modulating ligand, CAML, CAMLG

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using CAMLG Polyclonal antibody (23327-1-AP)

    WB analysis of Jurkat using 23327-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using CAMLG Polyclonal antibody (23327-1-AP)

    WB analysis of rat brain using 23327-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using CAMLG Polyclonal antibody (23327-1-AP)

    WB analysis of human testis using 23327-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CAMLG Polyclonal antibody (23327-1-AP)

    WB analysis of mouse testis using 23327-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CAMLG Polyclonal antibody (23327-1-AP)

    WB analysis of mouse brain using 23327-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23327-1-AP (CAMLG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CAMLG Polyclonal antibody (23327-1-AP)

    IHC staining of human breast cancer using 23327-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23327-1-AP (CAMLG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CAMLG Polyclonal antibody (23327-1-AP)

    IHC staining of human breast cancer using 23327-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23327-1-AP (CAMLG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, human testis tissue, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23327-1-AP is CAMLG Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAMLG fusion protein Ag19915 相同性解析による交差性が予測される生物種
Full Name calcium modulating ligand
Calculated molecular weight 296 aa, 33 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC130325
Gene symbol CAMLG
Gene ID (NCBI) 819
RRIDAB_2879256
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CAMLG antibody 23327-1-APDownload protocol
IHC protocol for CAMLG antibody 23327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

WEI (Verified Customer) (06-29-2020)

Sharp band at predicted size.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart