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Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CAMP Recombinant antibody (82940-2-RR)

    FC experiment of MCF-7 using 82940-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human CAMP (82940-2-RR, Clone:230157C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Hela cells using CAMP Recombinant antibody (82940-2-RR)

    FC experiment of Hela using 82940-2-RR

    (blue)1x10^6 Hela cells were intracellularly stained with 0.25 ug Anti-Human CAMP (82940-2-RR, Clone:230157C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution (red), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82940-2-RR

    Affinity and Kinetic Characterization of 82940-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82940-2-RR against Human CAMP were performed. The affinity constant is 1.50 nM.

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CAMP Recombinant antibody

CAMP Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 82940-2-RR
Clone No.230157C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

CAP18, Antibacterial peptide LL-23, Antibacterial peptide KS-30, Antibacterial peptide KR-20, Antibacterial peptide FF-33

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive FC (Intra) detected inMCF-7 cells, Hela cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82940-2-RR targets CAMP in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CAMP fusion protein Ag2622 相同性解析による交差性が予測される生物種
Full Name cathelicidin antimicrobial peptide
Calculated molecular weight 170 aa, 19 kDa
GenBank accession numberBC055089
Gene Symbol CAMP
Gene ID (NCBI) 820
RRIDAB_3670683
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49913
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.

Protocols

Product Specific Protocols
FC protocol for CAMP antibody 82940-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols