Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 10427-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 10427-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 10427-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10427-2-AP
WB result of Calnexin antibody (10427-2-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calnexin transfected HEK-293 cells.
WB analysis using 10427-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10427-2-AP (Calnexin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human cervical cancer using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human breast cancer using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human breast cancer using 10427-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10427-2-AP (CANX antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 10427-2-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was stained with CL594-Phalloidin (red).
IF Staining of SKOV-3 using 10427-2-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed SKOV-3 cells using Calnexin antibody (10427-2-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 10427-2-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of HepG2 using 10427-2-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10427-2-AP (Calnexin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10427-2-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 10427-2-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Calnexin (10427-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Calnexin Polyclonal antibody

Calnexin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 10427-2-AP

Publications(443)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Synonyms

CANX, P90, Major histocompatibility complex class I antigen-binding protein p88, IP90, CNX

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (443)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, U2OS cells, RAW 264.7 cells
Positive IHC detected in	human breast cancer tissue, human cervical cancer tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, SKOV-3 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 383 publications below
IHC	See 3 publications below
IF	See 64 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below

Product Information

10427-2-AP targets Calnexin in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, hamster, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Calnexin fusion protein Ag0697 相同性解析による交差性が予測される生物種
Full Name	calnexin
Calculated molecular weight	90 kDa
Observed molecular weight	90 kDa
GenBank accession number	BC003552
Gene Symbol	Calnexin
Gene ID (NCBI)	821
RRID	AB_2069033
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P27824
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Protocols

Product Specific Protocols
WB protocol for Calnexin antibody 10427-2-AP	Download protocol
IHC protocol for Calnexin antibody 10427-2-AP	Download protocol
IF protocol for Calnexin antibody 10427-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nature A virally encoded high-resolution screen of cytomegalovirus dependencies Authors - Yaara Finkel View Article
mouse	WB	Cell Res Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies Authors - Liang Guo View Article
human	WB	Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression Authors - Shiqiao Qiu View Article
human	WB	Cell Discov A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma Authors - Xifei Jiang View Article
		Immunity The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity Authors - Tian Chen View Article
human	WB	Nat Microbiol Modulation of membrane phosphoinositide dynamics by the phosphatidylinositide 4-kinase activity of the Legionella LepB effector. Authors - Na Dong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10427-2-AP

WB analysis using 10427-2-AP

WB analysis using 10427-2-AP

WB analysis of HEK-293 using 10427-2-AP

WB analysis using 10427-2-AP

IHC staining of human breast cancer using 10427-2-AP

IHC staining of human breast cancer using 10427-2-AP

IHC staining of human colon using 10427-2-AP

IHC staining of human cervical cancer using 10427-2-AP

IHC staining of human cervical cancer using 10427-2-AP

IHC staining of human breast cancer using 10427-2-AP

IHC staining of human breast cancer using 10427-2-AP

IF Staining of HepG2 using 10427-2-AP

IF Staining of SKOV-3 using 10427-2-AP

IF Staining of HepG2 using 10427-2-AP

IF Staining of HepG2 using 10427-2-AP

IF Staining of HepG2 using 10427-2-AP

FC experiment of HeLa using 10427-2-AP

Calnexin Polyclonal antibody

Calnexin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A virally encoded high-resolution screen of cytomegalovirus dependencies

Disruption of ER ion homeostasis maintained by an ER anion channel CLCC1 contributes to ALS-like pathologies

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

A RIPK3-independent role of MLKL in suppressing parthanatos promotes immune evasion in hepatocellular carcinoma

The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity

Modulation of membrane phosphoinositide dynamics by the phosphatidylinositide 4-kinase activity of the Legionella LepB effector.