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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CAPG Monoclonal antibody (66277-1-Ig)

    WB analysis of HeLa using 66277-1-Ig

    WB result of CAPG antibody (66277-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells.

  • Western Blot (WB) analysis of Jurkat cells using CAPG Monoclonal antibody (66277-1-Ig)

    WB analysis of Jurkat using 66277-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CAPG Monoclonal antibody (66277-1-Ig)

    WB analysis of HeLa using 66277-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CAPG Monoclonal antibody (66277-1-Ig)

    WB analysis of HL-60 using 66277-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66277-1-Ig (CAPG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CAPG Monoclonal antibody (66277-1-Ig)

    IHC staining of human lung cancer using 66277-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CAPG Monoclonal antibody (66277-1-Ig)

    IHC staining of human lung cancer using 66277-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66277-1-Ig (CAPG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CAPG Monoclonal antibody (66277-1-Ig)

    IF Staining of HeLa using 66277-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66277-1-Ig(CAPG antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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  • Featured Product
  • KD/KO Validated

CAPG Monoclonal antibody

CAPG Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66277-1-Ig
Clone No.3A5B2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HL-60 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66277-1-Ig targets CAPG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPG fusion protein Ag24676 相同性解析による交差性が予測される生物種
Full Name capping protein (actin filament), gelsolin-like
Calculated molecular weight 39 kDa
Observed molecular weight 39-40 kDa
GenBank accession numberBC000728
Gene Symbol CAPG
Gene ID (NCBI) 822
RRIDAB_2881660
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP40121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Protocols

Product Specific Protocols
WB protocol for CAPG antibody 66277-1-IgDownload protocol
IHC protocol for CAPG antibody 66277-1-IgDownload protocol
IF protocol for CAPG antibody 66277-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols